Review



human phosphor-src (tyr 419 ) elisa kit  (RayBiotech inc)


Bioz Verified Symbol RayBiotech inc is a verified supplier
Bioz Manufacturer Symbol RayBiotech inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    RayBiotech inc human phosphor-src (tyr 419 ) elisa kit
    NOX5 positively regulates <t>Src</t> activity. a Stable overexpression of NOX5 in the indicated ESCC cell lines tested by immunoblotting. GAPDH was used as a loading control. b Total protein lysates from the vector control or NOX5-overexpressing KYSE30 (upper panel) or KYSE410 (lower panel) cells were analyzed using antibody array against 43 kinase phosphorylation sites. c Silencing NOX5 in two specific short hairpin (sh) RNA-transduced stable ESCC cell lines examined by immunoblotting. GAPDH was used as a loading control. d vector control or NOX5-overexpressing KYSE30 (right panel) or KYSE410 (left panel) cells were pretreated with 10 μM NADPH oxidase inhibitor-DPI or control solvent for 90 min, respectively, or shRNA vector or NOX5 shRNA-1, shRNA-2, were cultured under normoxic and hypoxic conditions for 24 h. The Src activity was assayed by Src activation <t>quantitative</t> <t>ELISA</t> assay. e vector control or NOX5-overexpressing KYSE30 or KYSE410 cells were treated with ROS scavenger NAC (2 mM, pretreated with 90 min) or H 2 O 2 scavenger-PEG-catalase (400 units/ml) or control solvent, respectively, were cultured under normoxic and hypoxic conditions for 24 h. Src activity was assessed using quantitative ELISA assay. *** P < 0.001; two-tailed unpaired Student’s t -test. Error bars represent mean ± SD of five independent experiments. f NOX5 expression was associated with pSrc (Tyr 419 ), expression in 92 primary human ESCC specimens (cohort I). Two representative specimens with low and high levels of NOX5 were shown. Magnification, ×10 as indicated. Percentages of specimens showing low or high NOX5 expression relative to the level of pSrc. Statistical differences were evaluated using the chi-square test
    Human Phosphor Src (Tyr 419 ) Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phosphor-src+%28tyr+419+%29+elisa+kit/pmc07426961-209-19-26?v=RayBiotech+inc
    Average 90 stars, based on 1 article reviews
    human phosphor-src (tyr 419 ) elisa kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells"

    Article Title: Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells

    Journal: Signal Transduction and Targeted Therapy

    doi: 10.1038/s41392-020-0193-z

    NOX5 positively regulates Src activity. a Stable overexpression of NOX5 in the indicated ESCC cell lines tested by immunoblotting. GAPDH was used as a loading control. b Total protein lysates from the vector control or NOX5-overexpressing KYSE30 (upper panel) or KYSE410 (lower panel) cells were analyzed using antibody array against 43 kinase phosphorylation sites. c Silencing NOX5 in two specific short hairpin (sh) RNA-transduced stable ESCC cell lines examined by immunoblotting. GAPDH was used as a loading control. d vector control or NOX5-overexpressing KYSE30 (right panel) or KYSE410 (left panel) cells were pretreated with 10 μM NADPH oxidase inhibitor-DPI or control solvent for 90 min, respectively, or shRNA vector or NOX5 shRNA-1, shRNA-2, were cultured under normoxic and hypoxic conditions for 24 h. The Src activity was assayed by Src activation quantitative ELISA assay. e vector control or NOX5-overexpressing KYSE30 or KYSE410 cells were treated with ROS scavenger NAC (2 mM, pretreated with 90 min) or H 2 O 2 scavenger-PEG-catalase (400 units/ml) or control solvent, respectively, were cultured under normoxic and hypoxic conditions for 24 h. Src activity was assessed using quantitative ELISA assay. *** P < 0.001; two-tailed unpaired Student’s t -test. Error bars represent mean ± SD of five independent experiments. f NOX5 expression was associated with pSrc (Tyr 419 ), expression in 92 primary human ESCC specimens (cohort I). Two representative specimens with low and high levels of NOX5 were shown. Magnification, ×10 as indicated. Percentages of specimens showing low or high NOX5 expression relative to the level of pSrc. Statistical differences were evaluated using the chi-square test
    Figure Legend Snippet: NOX5 positively regulates Src activity. a Stable overexpression of NOX5 in the indicated ESCC cell lines tested by immunoblotting. GAPDH was used as a loading control. b Total protein lysates from the vector control or NOX5-overexpressing KYSE30 (upper panel) or KYSE410 (lower panel) cells were analyzed using antibody array against 43 kinase phosphorylation sites. c Silencing NOX5 in two specific short hairpin (sh) RNA-transduced stable ESCC cell lines examined by immunoblotting. GAPDH was used as a loading control. d vector control or NOX5-overexpressing KYSE30 (right panel) or KYSE410 (left panel) cells were pretreated with 10 μM NADPH oxidase inhibitor-DPI or control solvent for 90 min, respectively, or shRNA vector or NOX5 shRNA-1, shRNA-2, were cultured under normoxic and hypoxic conditions for 24 h. The Src activity was assayed by Src activation quantitative ELISA assay. e vector control or NOX5-overexpressing KYSE30 or KYSE410 cells were treated with ROS scavenger NAC (2 mM, pretreated with 90 min) or H 2 O 2 scavenger-PEG-catalase (400 units/ml) or control solvent, respectively, were cultured under normoxic and hypoxic conditions for 24 h. Src activity was assessed using quantitative ELISA assay. *** P < 0.001; two-tailed unpaired Student’s t -test. Error bars represent mean ± SD of five independent experiments. f NOX5 expression was associated with pSrc (Tyr 419 ), expression in 92 primary human ESCC specimens (cohort I). Two representative specimens with low and high levels of NOX5 were shown. Magnification, ×10 as indicated. Percentages of specimens showing low or high NOX5 expression relative to the level of pSrc. Statistical differences were evaluated using the chi-square test

    Techniques Used: Activity Assay, Over Expression, Western Blot, Plasmid Preparation, Ab Array, shRNA, Cell Culture, Activation Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Expressing



    Similar Products

    human phosphor-src (tyr 419 ) elisa kit  (RayBiotech inc)
    90
    RayBiotech inc human phosphor-src (tyr 419 ) elisa kit
    NOX5 positively regulates <t>Src</t> activity. a Stable overexpression of NOX5 in the indicated ESCC cell lines tested by immunoblotting. GAPDH was used as a loading control. b Total protein lysates from the vector control or NOX5-overexpressing KYSE30 (upper panel) or KYSE410 (lower panel) cells were analyzed using antibody array against 43 kinase phosphorylation sites. c Silencing NOX5 in two specific short hairpin (sh) RNA-transduced stable ESCC cell lines examined by immunoblotting. GAPDH was used as a loading control. d vector control or NOX5-overexpressing KYSE30 (right panel) or KYSE410 (left panel) cells were pretreated with 10 μM NADPH oxidase inhibitor-DPI or control solvent for 90 min, respectively, or shRNA vector or NOX5 shRNA-1, shRNA-2, were cultured under normoxic and hypoxic conditions for 24 h. The Src activity was assayed by Src activation <t>quantitative</t> <t>ELISA</t> assay. e vector control or NOX5-overexpressing KYSE30 or KYSE410 cells were treated with ROS scavenger NAC (2 mM, pretreated with 90 min) or H 2 O 2 scavenger-PEG-catalase (400 units/ml) or control solvent, respectively, were cultured under normoxic and hypoxic conditions for 24 h. Src activity was assessed using quantitative ELISA assay. *** P < 0.001; two-tailed unpaired Student’s t -test. Error bars represent mean ± SD of five independent experiments. f NOX5 expression was associated with pSrc (Tyr 419 ), expression in 92 primary human ESCC specimens (cohort I). Two representative specimens with low and high levels of NOX5 were shown. Magnification, ×10 as indicated. Percentages of specimens showing low or high NOX5 expression relative to the level of pSrc. Statistical differences were evaluated using the chi-square test
    Human Phosphor Src (Tyr 419 ) Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phosphor-src+%28tyr+419+%29+elisa+kit/pmc07426961-209-19-26?v=RayBiotech+inc
    Average 90 stars, based on 1 article reviews
    human phosphor-src (tyr 419 ) elisa kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    NOX5 positively regulates Src activity. a Stable overexpression of NOX5 in the indicated ESCC cell lines tested by immunoblotting. GAPDH was used as a loading control. b Total protein lysates from the vector control or NOX5-overexpressing KYSE30 (upper panel) or KYSE410 (lower panel) cells were analyzed using antibody array against 43 kinase phosphorylation sites. c Silencing NOX5 in two specific short hairpin (sh) RNA-transduced stable ESCC cell lines examined by immunoblotting. GAPDH was used as a loading control. d vector control or NOX5-overexpressing KYSE30 (right panel) or KYSE410 (left panel) cells were pretreated with 10 μM NADPH oxidase inhibitor-DPI or control solvent for 90 min, respectively, or shRNA vector or NOX5 shRNA-1, shRNA-2, were cultured under normoxic and hypoxic conditions for 24 h. The Src activity was assayed by Src activation quantitative ELISA assay. e vector control or NOX5-overexpressing KYSE30 or KYSE410 cells were treated with ROS scavenger NAC (2 mM, pretreated with 90 min) or H 2 O 2 scavenger-PEG-catalase (400 units/ml) or control solvent, respectively, were cultured under normoxic and hypoxic conditions for 24 h. Src activity was assessed using quantitative ELISA assay. *** P < 0.001; two-tailed unpaired Student’s t -test. Error bars represent mean ± SD of five independent experiments. f NOX5 expression was associated with pSrc (Tyr 419 ), expression in 92 primary human ESCC specimens (cohort I). Two representative specimens with low and high levels of NOX5 were shown. Magnification, ×10 as indicated. Percentages of specimens showing low or high NOX5 expression relative to the level of pSrc. Statistical differences were evaluated using the chi-square test

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells

    doi: 10.1038/s41392-020-0193-z

    Figure Lengend Snippet: NOX5 positively regulates Src activity. a Stable overexpression of NOX5 in the indicated ESCC cell lines tested by immunoblotting. GAPDH was used as a loading control. b Total protein lysates from the vector control or NOX5-overexpressing KYSE30 (upper panel) or KYSE410 (lower panel) cells were analyzed using antibody array against 43 kinase phosphorylation sites. c Silencing NOX5 in two specific short hairpin (sh) RNA-transduced stable ESCC cell lines examined by immunoblotting. GAPDH was used as a loading control. d vector control or NOX5-overexpressing KYSE30 (right panel) or KYSE410 (left panel) cells were pretreated with 10 μM NADPH oxidase inhibitor-DPI or control solvent for 90 min, respectively, or shRNA vector or NOX5 shRNA-1, shRNA-2, were cultured under normoxic and hypoxic conditions for 24 h. The Src activity was assayed by Src activation quantitative ELISA assay. e vector control or NOX5-overexpressing KYSE30 or KYSE410 cells were treated with ROS scavenger NAC (2 mM, pretreated with 90 min) or H 2 O 2 scavenger-PEG-catalase (400 units/ml) or control solvent, respectively, were cultured under normoxic and hypoxic conditions for 24 h. Src activity was assessed using quantitative ELISA assay. *** P < 0.001; two-tailed unpaired Student’s t -test. Error bars represent mean ± SD of five independent experiments. f NOX5 expression was associated with pSrc (Tyr 419 ), expression in 92 primary human ESCC specimens (cohort I). Two representative specimens with low and high levels of NOX5 were shown. Magnification, ×10 as indicated. Percentages of specimens showing low or high NOX5 expression relative to the level of pSrc. Statistical differences were evaluated using the chi-square test

    Article Snippet: Levels of activated Src and Pyk2 in the cell lysis (~20 μg protein per sample) were measured using the human phosphor-Src (Tyr 419 ) ELISA kit (Raybiotech; catalog# PEL-SRC-Y419-T) and the human phosphor-Pyk2 (Tyr 402 ) ELISA kit (Raybiotech; catalog# PEL-PYK2-Y402-T) following the manufacturer’s protocols.

    Techniques: Activity Assay, Over Expression, Western Blot, Plasmid Preparation, Ab Array, shRNA, Cell Culture, Activation Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Expressing